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KMID : 1140120120170020121
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Cancer Prevention Research
2012 Volume.17 No. 2 p.121 ~ p.127
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Regulation of TNF-α, Akt and COX-2 by Resveratrol in MCF-7 Breast Cancer Cells
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Lee Sol-Hwa
Seo Dong-Ik
Kim Hye-Seon
Park Song-Yi
Park Ock-Jin
Kim Young-Min
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Abstract
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Resveratrol, a naturally occurring polyphenol, has broad-spectrum beneficial effects including antiproliferation, anti-inflammatory and anti-cancer activities. TNF-α is highly expressed in cancer cells. Akt is also highly expressed in breast cancer and regulates cell proliferation and survival. COX-2 is increased by prostaglandins, which includes cell proliferation and angiogenesis. In this study, we explored the anti-proliferative effects of resveratrol via inhibition of TNF-α, Akt and COX-2 in MCF-7 breast cancer cells. In MTT assay, resveratrol inhibits proliferation of MCF-7 breast cancer cells in does-dependent and time-dependent manner. Resveratrol also induced apoptotic cell death of cancer cells. Resveratrol decreased anti-apoptotic protein (Bcl-2) and increased pro-apoptotic protein (Bax, caspase-3 and PARP). Treatment with resveratrol decreased TNF-α in cytosol and nucleus, reduced Akt and COX-2 expression in the nucleus on MCF-7 breast cancer cell. These results suggest that resveratrol induced apoptosis through inhibition of TNF-α, Akt and COX-2 in MCF-7 breast cancer cells.
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KEYWORD
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Resveratrol, TNF-α, Akt, COX-2, MCF-7 breast cancer cell
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